Peptide Nucleic Acids

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Peptide Nucleic Acids

Author : Peter E. Nielsen
ISBN : 9781592592906
Genre : Science
File Size : 30.4 MB
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Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process. Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development.
Category: Science

Peptide Nucleic Acids

Author : Peter E. Nielsen
ISBN : 0954523245
Genre : Science
File Size : 64.51 MB
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Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.
Category: Science

Peptide Nucleic Acids

Author : Eylon Yavin
ISBN : 3039368869
Genre :
File Size : 48.41 MB
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This book compiles four review articles and four research papers that highlight recent developments in the field of peptide nucleic acid (PNA) chemistry and biomedicine. The review articles encompass a variety of fields related to PNA, emphasizing the versatility of this DNA mimic. Two excellent reviews detail the use of PNA for molecular diagnostics of miRNAs and genetic point mutations (SNPs). Another review provides a comprehensive analysis of the various approaches for gene editing using chemically modified PNA molecules. Lastly, PNA molecules are elegantly described as effective (antisense) antimicrobial agents in the final review. The high binding affinity of PNA to complementary DNA and RNA is highlighted in three research articles. Two articles show how PNA molecules act as splice modulating and RNA masking molecules, separately. In another contribution, the high affinity and achiral characteristics of PNAs are used to developed a stable L-DNA-based catalytic hairpin assembly. Lastly, chemically-modified PNA molecules are shown to be superior probes for SNP detection. Altogether, these studies illustrate how PNA molecules may be useful for a variety of biomedical applications as either therapeutic or diagnostic agents.
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Peptide Nucleic Acids Morpholinos And Related Antisense Biomolecules

Author : Christopher Janson
ISBN : 9780387329567
Genre : Medical
File Size : 58.96 MB
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This volume is unique to the existing literature in the Peptide Nucleic Acid field, in that it focuses on comparing and contrasting PNA with other available oligonucleotide homologues and considers areas in which these biomolecules could be profitably applied to clinical and diagnostic applications. Part I of the book addresses comparative strengths and weaknesses of various nucleoside homologues. Part II of the book addresses specific translational or clinical applications for PNA and related antisense biomolecules. The editors have succeeded in presenting a balanced yet broad view of the methods available for gene targeting and modification.
Category: Medical

Peptide Nucleic Acids And Markers

Author : Prasanna Ramani
ISBN : 384432805X
Genre :
File Size : 70.37 MB
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The remarkable binding properties of 'Peptide Nucleic Acids' (PNA), the so called artificial DNAs, have spawned a rapidly expanding new field of research, where the targets are the synthesis of PNAs and PNA analogues, and their applications as therapeutics, DNA diagnostics, tools in biotechnology and much more. PNA is linked to various scientific disciplines, few includes medicinal chemistry, biochemistry, molecular biology, immunology and drug development. This book describes the design, synthesis and analytical studies of labeled nucleic acid analog. Basic methods for the synthesis of PNA monomer/oligomer are incorporated. Further, the syntheses of electrochemical and fluorescently labeled PNA monomers/oligomers are dealt-with in detail. The final part of the book discusses about the possibility of using linkers/spacers to carry marker to link PNA monomer/oligomer. This book would be an inspiration for undergraduates/postgraduates and experienced researchers as it provides an easy introduction to the optimal use of PNA in various disciplines.
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Peptide Nucleic Acids

Author : Peter E. Nielsen
ISBN : 1898486379
Genre : Nucleic acids
File Size : 87.39 MB
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Category: Nucleic acids

Evaluation Of The Protein Recognition Properties Of Peptide Nucleic Acids

Author : Crystal Cox Serrano
ISBN : OCLC:951102390
Genre : Nucleic acids
File Size : 51.7 MB
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The objective is to evaluate the ability of aminoacyl-tRNA synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in many biomedical applications due to its specificity, strong binding, and nuclease resistance. This study is the initial data set that indicates PNA are recognized by translation enzymes.
Category: Nucleic acids

Side Chain

Author : Ramin Hamzavi
ISBN : OCLC:488961451
Genre :
File Size : 80.52 MB
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Design Synthesis And Physicochemical Properties Of Aromatic Peptide Nucleic Acids

Author : Lee David Fader
ISBN : OCLC:61568216
Genre :
File Size : 57.33 MB
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"Synthetic methods were developed for the preparation of several structurally diverse aromatic peptide nucleic acid (APNA) monomers containing all four natural DNA bases. This set of building blocks was useful for the preparation of oligomers designed to evaluate the hybridization properties of novel peptide nucleic acid (PNA) analogues, which incorporate aromatic rings into their backbone. Protocols for the solid-phase synthesis of APNA-PNA chimeras and APNA homopolymers were also developed." --
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Pseudo Peptides In Drug Discovery

Author : Peter E. Nielsen
ISBN : 9783527605699
Genre : Science
File Size : 80.46 MB
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Peptides are among the most versatile bioactive molecules, yet the do not make good drugs, because they are quickly degraded or modified in the body. To overcome this problem, stable and at the same time biologically active pseudo-peptides have been developed. These novel compounds open up new perspectives in drug design by providing an entire range of highly specific and non-toxic pharmaceuticals. This is the first work devoted to the topic and draws together knowledge gained on different types of peptidomimetics and other pseudo-peptides with drug properties. As such, it includes peptoids, beta-peptides, polyamide DNA binders as well as peptide nucleic acids. The expert authors and editor discuss chemical properties and stability, biological activity and reactivity, as well as practical aspects of synthesis, making this a prime resource for drug developers and bioorganic chemists working with these compounds.
Category: Science